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June 08, 2020

Genetics by

Four coding sequences encoding the key enzymes involved in Panax notoginseng saponin biosynthesis were cloned: farnesyl diphosphate synthase, squalene synthase, squalene epoxidase, and dammarenediol-II synthase. Xia 2019

Protopanaxadiol and protopanaxatriol-type saponins in Panax notoginseng root tissues were found to be higher than above ground tissues, while CYP716A47 and CYP716A53v2 expression patterns were correlated with distribution of protopanaxadiol-type and protopanaxatriol-type saponins, respectively. Wei 2018

The complete nucleotide sequence of the Panax notoginseng chloroplast genome, built using next-generation sequencing technology, is presented. Phylogenomic analysis showed P. notoginseng to be closely related to P. ginseng. Zhang 2016

Candidate genes involved in ginsenoside biosynthesis, including putative cytochrome P450s and glycosyltransferases, were identified from the Panax notoginseng transcriptome using next generation DNA sequencing. Liu 2015

Phylogenetic and population genetic analyses, coupled with species distribution patterns of Panax, suggest that two rounds of whole genome duplication along with geographic and ecological isolation may have contributed to the evolution and diversification of the Panax genus. Shi 2015

Novel and conserved microRNAs in 1-, 2-, and 3-year-old Panax notoginseng roots were analyzed via next-gen high-throughput sequencing, identifying a large number of miRNAs and their target genes, functional annotations, and gene expression patterns. Wei 2015

Normal expression of 14 candidate reference genes in Panax notoginseng were characterized with primer specificity and amplification efficiency evaluated for each gene and candidates subjected to tissue-specific transcript quantification. Wu 2015

70 expressed sequence tag-derived polymorphic simple sequence repeat markers by trials of 140 primer pairs were developed to analyze genetic diversity of Panax ginseng cultivars. Phylogenetic analysis showed 3 distinctive groups: P. ginseng-P. japonicus clade, P. notoginseng, and P. quinquefolius. Choi 2011

Twelve polymorphic microsatellite loci were isolated and characterized from the microsatellite-enriched genomic library of Panax notoginseng. Liu 2011

Putative triterpene saponin-biosynthetic genes and genetic markers were identified and characterized by next-generation sequencing technology for the Panax notoginseng root transcriptome. In addition, identification of SSRs provided genetic makers for molecular breeding and genetics applications. Luo 2011

The genetic diversity and structure of 17 breeding strains of Panax notoginseng were analyzed using the EST-SSR molecular marker, with a total of 136 polymorphic loci of EST-SSR detected. [Article in Chinese] Zhang 2011

Panax notoginseng was found to be identified from 4 medicines of Panax L. genus (Panax ginseng C. A. Meyer, P. quinquefolicum L. , P. notoginseng (Burk.) F. H. Chen, and P. japonicus C. A. Meyer) based on the sequence difference in the mitochondrial nad 1 gene. [Article in Chinese] Gu 2010

cDNA libraries constructed using suppression subtractive hybridization found 110 genes to be highly expressed in 3-year-old Panax notoginseng roots and 80 genes in 1-year-old roots with global gene expression profiles showing significant differences between the 1- and 3-year-old roots. He 2008

Protopanaxadiol 6-hydroxylase was found to be a key enzyme in regulating Rg-group ginsenoside biosynthesis in Panax notoginseng cells. Yue 2008

The nuclear 18S rRNA and chloroplast MATK genes of 18 samples of Panax notoginseng and its processed material Sanqi (Radix Notoginseng) were analyzed with the two genes, regardless of cultivar origin, found to be identical to genotype R1 and M1, respectively, of the published sequences. Zhang 2006